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The role of "boundary conditions" in the 3D genome architecture


3D nuclear architecture is vital to genome function, however its underlying principles are only beginning to emerge. This collaborative project (with I. Sharakhov, entomology) aims to uncover the fundamental mechanisms of maintaining nuclear architecture via interactions between chromosomes and the nuclear envelope (NE) – an area almost unexplored. Specifically, investigate the role of the “boundary conditions” -- chromosome–NE attachments -- in establishing intra- and inter-chromosomal contacts. Solving this problem will significantly improve our understanding of the role of nuclear architecture in regulation of gene expression, DNA double-strand break repair, generation of chromosomal rearrangements, and, ultimately, epigenetic inheritance. Modeling of giant (polytene) chromosomes in fruit fly